yugJ [2019-07-09 11:33:21]

similar to NADH-dependent butanol dehydrogenase

locus: BSU31370

pI: 5.22

mw: 42.57 kDa

protein length: 387 aa

gene length: 1164 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,223,471 3,224,634

The protein

Protein family

• iron-containing alcohol dehydrogenase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|D81610C7CA2A92FCB1195591ACB613E7C1288F6B|YugK]

Structure

• [PDB|1VLJ] (from Thermotoga maritima, 44% identity)

Expression and Regulation

Operons

• genes: [gene|1E951C2B640966643D5F081159522CD1C51942C8|yugJ]
  description: [Pubmed|9274030]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|14597697,19575568], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

Biological materials

Mutant

• MGNA-B549 (yugJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1548 NBRP B. subtilis, Japan]
• BKE31370 ([gene|1E951C2B640966643D5F081159522CD1C51942C8|yugJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACATCTCCTCCTTAGA, downstream forward: _UP4_TAAAAAAAGGGGAAATAGCC
• BKK31370 ([gene|1E951C2B640966643D5F081159522CD1C51942C8|yugJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACATCTCCTCCTTAGA, downstream forward: _UP4_TAAAAAAAGGGGAAATAGCC

References

Reviews

• 20924577

Original publications

• 9274030

The corresponding protein in ''E. coli

• 20543070,18211903